For customers with Xevo TQ-S micro and Xevo TQ-XS mass spectrometers running on MassLynx™ Software version 4.2, Waters is introducing a new interface to Skyline software, the freely available, open source software for targeted proteomics. The interface will enable scientists to develop optimised LC-MS/MS multiple reaction monitoring (MRM) methods for the quantification of peptides or protein digests by the surrogate peptide approach. The MassLynx/Skyline interface is an easy-to-use tool for auto-optimising and fine-tuning high-sensitivity MRM assays on tandem quadrupole mass spectrometers for bioanalytical or targeted proteomics experiments that are part of drug discovery research. The MassLynx/Skyline interface can be downloaded for free from the Waters Marketplace.