The Thermo Scientific Orbitrap IQ-X Tribrid mass spectrometer has been designed for small-molecule structural elucidation of metabolites and unknown compounds. The instrument utilises Thermo Scientific AcquireX workflows for increased sample throughput and ease-of-use. It provides improved fragmentation for small-molecule analysis with options to add to the platform’s experimental flexibility. An ultraviolet photodissociation option provides insights on the lipid double-bond localisation, site-specific glucuronidation and complementary fragmentation for structure assignment while the 1,000,000 resolution option improves unknown analysis and fine isotope detection.